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Data sheet
| Formulation | PSMA6 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 40% glycerol. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Description | PSMA6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 270 amino acids (1-246 a.a.) and having a molecular mass of 29.9kDa.PSMA6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | PSMA6 belongs to the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with an extremely organized ring-shaped 20S core structure. The core structure consists of 4 rings of 28 non-identical subunits; 2 rings consist of 7 alpha subunits and 2 rings consist of 7 beta subunits. PSMA6 is spread all over eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent procedure in a non-lysosomal pathway. |
| Expression host | Escherichia Coli. |
| Synonyms | Proteasome (prosome, macropain) subunit alpha type 6, PROS27, p27K, IOTA, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, 27 kDa prosomal protein. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTVTHLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD |
More info
27 kDa prosomal protein, IOTA, Macropain iota chain, macropain) subunit alpha type 6, Multicatalytic endopeptidase complex iota chain, p27K, PROS27, Proteasome iota chain, Proteasome (prosome