Data sheet
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50mM Phosphate buffer pH=7.1 and 150mM NaCl. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Interleukin-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton.The IL-1b is purified by proprietary chromatographic techniques. |
| Protein Background | Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Expression host | Escherichia Coli. |
| Synonyms | Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser. |
| Biological Activity | The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg. |
| References | 1.Title: Proinflammatory Cytokine IL-1b Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis. Publication: Published online before print March 1, 2010, doi: 10.4049/ jimmunol.0901735 The Journal of Immunology April 1, 2010 vol. 184 no. 7 3946-3954. Link: http://www.jimmunol.org/content/184/7/3946.full Application: Level of IL-8 mRNA was dramatically enhanced by IL-1b,HMC-1 cells were stimulated with IL-1b. 2.Title:Contribution of IL-1 to resistance to Streptococcus pneumoniae infection .Publication: Int. Immunol. (2008) 20 (9): 1139-1146. doi: 10.1093/intimm/dxn071 First published online: July 1, 2008 Link:http://intimm.oxfordjournals.org/content/20/9/1139.full3.Title: Hydrogen sulphide decreases IL-1b induced activation of fibroblast?like synoviocytes from patients with osteoarthritisPublications: journal of cellular and molecular medicine 19.1 (2015): 187-197Link: http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12405/epdf 4. Title: Rifampin Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial CellsPublications: Antimicrobial agents and chemotherapy 50.1 (2006): 396-398Link: http://aac.asm.org/content/50/1/396.full5. Title: Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediatorsPublication: Arthritis Res Ther 11.6 (2009): R161.Link: http://www.biomedcentral.com/content/pdf/ar2844.pdf |
More info
Endogenous Pyrogen (EP), IL-1 beta, IL1F2, Leukocyte Endogenous Mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF), Catabolin