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Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | CD34 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 20% glycerol and 1mM DTT. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | CD34 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (32-290a.a.) and having a molecular mass of 29.7kDa. CD34 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | CD34 is a single chain transmembrane glycoprotein which is selectively expressed on human lymphoid and myeloid hemapoietic progenitor cells.CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Expression host | E.coli. |
| Synonyms | CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSLDNNGTAT PELPTQGTFS NVSTNVSYQE TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT. |
| Antibody Properties | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates | Cytotoxic and staining antibody. For staining, use 10ul/1,000,000 cells. Exact titer for cytotoxicity should be determined by the investigator. |
| Immunological function | This antibody is only available as purified antibody. |
More info
CD34, Hematopoietic progenitor cell antigen CD34