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Adiponectin Human Recombinant ( Acrp30 Human )
Patent RightsThe sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).DescriptionThe Adiponectin Human recombinant protein is a single, non-glycosilated polypeptide chain produced in E. coli,
Data sheet
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Formulation | Acrp30 Human was filtered (0.05um) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS, 50mM NaCl and 1mM CaCl2, pH 7.5 |
| Solubility | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. |
| Purity | Acrp30 purity is greater than 90% as determined by SDS-PAGE. |
| Applications | Cell culture and/or animal studies, ELISA, Immunological methods and Western blotting |
| Description | The Acrp30 Human protein contains 244 amino acid having a molecular mass of 26.42kDa. Native adiponectin is isolated from human pooled sera and contains all HMW, MMW and LMW fractions. |
| Protein Background | Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. |
| Expression host | Human pooled serum |
| Synonyms | Acrp30, AdipoQ, GBP-28, APM-1, ACDC. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Amino acid sequence | MLLLGAVLLL LALPGHDQET TTQGPGVLLP LPKGACTGWM AGIPGHPGHN GAPGRDGRDG TPGEKGEKGD PGLIGPKGDI GETGVPGAEG PRGFPGIQGR KGEPGEGAYV YRSAFSVGLE TYVTIPNMPI RFTKIFYNQQ NHYDGSTGKF HCNIPGLYYF AYHITVYMKD VKVSLFKKDK AMLFTYDQYQ ENNVDQASGS VLLHLEVGDQ VWLQVYGEGE RNGLYADNDN DSTFTGFLLY HDTN |