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Alanyl-tRNA Synthetase Human Recombinant ( PL 12 Human )

DescriptionAlanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.SourceSf9

$193.00

Data sheet

Formulation PL-12 is supplied in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.
Purity Greater than 90% as determined by SDS-PAGE.
Description Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Protein Background Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
Expression host Sf9 insect cells.
Synonyms Alanyl-tRNA synthetase cytoplasmic, EC 6.1.1.7, Alanine-tRNA ligase, AlaRS, Renal carcinoma antigen NY-REN-42, PL-12, AARS.
Reagent Appearance Sterile Filtered clear solution.
Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Immunological functions 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Coating concentration 0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

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