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Modified Affinity Purified Avidin ( Avidin Biotin )
DescriptionAvidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and
Data sheet
| Formulation | Salt-free lyophilized powder with no preservative added. |
| Solubility | We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved. |
| Description | Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii. |
| Synonyms | Avidin, AVD, AVID. |
| Reagent Appearance | Lyophilized freeze dry Powder. |
| Stability | Store protein solution at 2-8°C. |
| Activity | Binding activity of Avidin-Biotin is 10-15units/mg protein. One unit of Avidin activity is defined as the amount of protein which will bind 1ug d-biotin. |