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Beta-Nerve Growth Factor Human Recombinant, CHO ( b NGF Human, CHO )
DescriptionNerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.The NGF-b is purified
Data sheet
| Formulation | The protein was lyophilized from a 0.2um filtered solution in 20mM PB and 250mM NaCl, pH 7.0. |
| Solubility | It is recommended to reconstitute the lyophilized NGF-b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Nerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.The NGF-b is purified by proprietary chromatographic techniques. |
| Protein Background | NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. |
| Expression host | Chinese Hamster Ovary Cells. |
| Synonyms | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | Was analyzed by Mass spectrometry. |
| Biological Activity | The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be 1 x 106 units/mg. |