View larger
Human Calpain-1 Catalytic Subunit ( CAPN1 )
DescriptionCAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit. CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.SourceHuman Erythrocytes.Physical
Data sheet
| Formulation | 50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | CAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit. CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns. |
| Protein Background | Calpains activity is attributed to two main isoforms: ?-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (?-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin. |
| Expression host | Human Erythrocytes. |
| Synonyms | Calpain-1 catalytic subunit, EC 3.4.22.52, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, muCANP, Micromolar-calpain, Cell proliferation-inducing gene 30 protein, CANP 1, CAPN1, CANPL1, PIG30, CANP, muCL, CANP1. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | CAPN1 although stable at 10°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |