View larger
Carbonyl Reductase-4 Human Recombinant ( CBR4 Human )
DescriptionCBR4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 257 amino acids (1-237 a.a.) and having a molecular mass of 27.5kDa. The CBR4 is
Data sheet
| Specificity | CD31 is expressed in high levels on endothelial cells and in lower levels on monocytes, granulocytes and platelets. |
| Formulation | The CBR4 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 5mM DTT and 200mM NaCl. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | CBR4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 257 amino acids (1-237 a.a.) and having a molecular mass of 27.5kDa. The CBR4 is purified by proprietary chromatographic techniques. |
| Protein Background | CBR4 is a member of the short-chain dehydrogenase/reductase family. CBR4 has a role in biosynthesis of fatty acids in the mitochondria and a broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and a range of other o-quinones and p-quinones (in vitro). CBR4 formation of a heteroteramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones. |
| Expression host | Escherichia Coli. |
| Synonyms | Carbonyl reductase family member 4, 3-oxoacyl-[acyl-carrier-protein] reductase, Quinone reductase CBR4, CBR4, SDR45C1, FLJ14431. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL. |
| Antibody Properties | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Immunological function | This antibody is also available conjugated to FITC and biotin. For flow cytometry use 5-10ul/106 cells. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |