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Calcium and Integrin Binding 1 Human Recombinant ( CIB1 Human )
DescriptionCIB1 Human Recombinant is a single, non-glycosylated polypeptide chain containing 211 amino acids and having a molecular mass of 23 kDa. CIB1 was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by
Data sheet
| Formulation | The CIB1 protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8 & 5mM DTT. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | CIB1 Human Recombinant is a single, non-glycosylated polypeptide chain (aa 1-191) containing a total of 211 amino acids and having a molecular mass of 23 kDa.CIB1 is fused to a 20 a.a His-tag and it was purified by conventional column chromatographic techniques. |
| Protein Background | CIB1 (Calcium and integrin binding 1) is regulatory protein with 50% homology to calmodulin and calcineurin B, that encodes a member of the calcium-binding protein family. CIB1 interacts with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. Also CIB1 is widely expressed and binds to a number of effectors, such as integrin ?IIb, PAK1, and polo-like kinases, in different tissues. |
| Expression host | Escherichia Coli. |
| Synonyms | Calcium and integrin-binding protein 1, Calmyrin, DNA-PKcs-interacting protein, Kinase-interacting protein, SNK-interacting protein 2-28, SIP2-28, CIB1, CIB, KIP, PRKDCIP. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a 0.1% HSA or BSA. Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGGSGSRLSK ELLAEYQDLT FLTKQEILLA HRRFCELLPQ EQRTVESSLR AQVPFEQILS LPELKANPFK ERICRVFSTS PAKDSLSFED FLDLLSVFSD TATPDIKSHY AFRIFDFDDD GTLNREDLSR LVNCLTGEGE DTRLSASEMK QLIDNILEES DIDRDGTINL SEFQHVISRS PDFASSFKIV L. |
| Advantages | Plasma from each donor has been tested and found negative for HIV-1 & 2 antibodies, Hepatatis B surface antigen, and Hepatatis C antibodies. |