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Casein Kinase 2 Holoenzyme Human Recombinant ( CK2h Human )
DescriptionHuman recombinant casein kinase 2 holo enzyme containing alpha and beta subunits which were separately expressed in E. coli as non-fusion proteins and purified using several chromatographic steps. The holo enzyme has been reconstituted in
Data sheet
| Formulation | CK2 Holoenzyme is supplied 0.5mg/1ml in 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500 uM PMSF, 5% glycerol, pH 8.5. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | Human recombinant casein kinase 2 holo enzyme containing alpha and beta subunits which were separately expressed in E. coli as non-fusion proteins and purified using several chromatographic steps. The holo enzyme has been reconstituted in the course of the purification and is highly active suitable for labelling CK2 substrates. CK2 holoenzyme is a non-glycosilated polypeptide having a molecular mass of 140 kDa. |
| Expression host | Escherichia Coli. |
| Synonyms | Casein Kinase 2 Holoenzyme, CK2h. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Unit Definition | No protease activity detectable, specific activity > 1.3U/mg (1U = 1umol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles. |