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Creatine Kinase MB Isoenzyme Type-II Human Recombinant ( CKMBITII Human )
DescriptionCKMBITII Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal
Data sheet
| Formulation | Each mg of protein contains 20mM Tris-HCl, pH-6.8, 1mM EDTA and 1mM DTT, 50% (v/v) glycerol. |
| Description | CKMBITII Human Recombinant produced in Pichia Pastoris reacts with polyclonal antibodies to MB Isoenzyme in ELISA. |
| Protein Background | CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine.This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI. |
| Expression host | Pichia Pastoris. |
| Synonyms | Creatine Kinase MB Isoenzyme Type-II, CKMBITII, CKMBI, CKMB. |
| Reagent Appearance | Sterile Filtered colourless liquid formulation. |
| Stability | CKMBITII although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles. |
| Biological Activity | The enzyme activity measured by kinetic assay at 340nm was 883 IU/mg at 37 degrees Celsius, corresponding to a Specific Activity of 1,133ng/ml. |