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Creatine Kinase MB Isoenzyme Type-I Human Recombinant ( CKMBITI Human )
DescriptionCKMBITI Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies
Data sheet
| Formulation | The 7.9mg/ml protein solution contains 0.02M Potassium Phosphate, 1mM DTT and 50% glycerol, pH 5.0-6.0. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | CKMBITI Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain and a full length Creatine Kinase MB isoenzyme, which is missing the C-terminal Lysine on the M subunit. CKMBITI is a dimeric protein comprised of M and B subunits, having a total Mw of ~44kDa. The CKMBITI is purified by proprietary chromatographic techniques. |
| Protein Background | The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases. |
| Expression host | Pichia Pastoris. |
| Synonyms | Creatine Kinase MB Isoenzyme Type-I, CKMBITI, CKMBI, CKMB. |
| Reagent Appearance | Sterile Filtered colorless liquid formulation. |
| Stability | CKMBITI although stable at 15°C for 7 days, should be stored below -18°C.Please prevent freeze-thaw cycles. |
| Biological Activity | 486 IU/mg at 37°C. |