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Dengue Virus Subtype 1 & 3 fused Envelope 58kDa, Suitable for Gold Conjugation Recombinant ( Dengue Envelope-1 & 3 )
DescriptionThe E.coli derived recombinant 58kDa protein is a genetically engineered peptide which is derived from Dengue Type-1 and 3 to be expressed as a fused envelope, it was designed specifically for gold conjugation in a rapid test production,
Data sheet
| Formulation | The protein solution contains Phosphate buffered saline, pH-7.4. |
| Purity | Protein is >95% pure as determined by 12% PAGE (coomassie staining). |
| Description | The E.coli derived recombinant 58kDa protein is a genetically engineered peptide which is derived from Dengue Type-1 and 3 to be expressed as a fused envelope, each part in this fusion contains 170 a.a (positions 46-217), it is used in ELISA assay. This fusion protein is connected to a 6xHis Tag. Dengue Type-1 and 3 is purified by proprietary chromatographic technique. |
| Protein Background | Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus. |
| Expression host | Escherichia Coli. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Dengue Envelope-1 & 3 Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Peptide Content | - Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37°C. 1. Add the reaction buffer to each well.2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.3. Add the enzyme (DP |