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Dihydrolipoamide S-Succinyltransferase Human Recombinant ( DLST Human )
PT_74356
DescriptionDLST is a Full-length cDNA coding for the mature form of the human OGDC-E2 protein having a molecular mass of 42,301 Dalton (pH 6.3).DLST protein is fused to a hexa-histidine purification tag.SourceSf9 insect cells.FormulationDLST
Data sheet
| Formulation | DLST (0.74mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol. |
| Purity | DLST purity was found to be greater than 75% as determined by SDS-PAGE. |
| Applications | Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody. |
| Description | DLST is a Full-length cDNA coding for the mature form of the human OGDC-E2 protein having a molecular mass of 42,301 Dalton (pH 6.3).DLST protein is fused to a hexa-histidine purification tag. |
| Protein Background | DLST catalyzes the general transformation of 2-oxoglutarate to succinyl-CoA and CO(2). DLST holds multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Expression host | Sf9 insect cells. |
| Synonyms | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex), Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, component E2, DLST, E2K, OGDC-E2, EC 2.3.1.61, EC 2.3.1. |
| Storage Conditions | Store DLST at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Coating concentration | 0.4-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |