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H1N1 Influenza-A Virus Solomon Islands/03/06 Recombinant ( H1N1 Solomon Islands )
DescriptionRecombinant Full-Length H1N1 A/Solomon Islands/03/2006 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells.SourceBaculovirus Insect Cells.Physical AppearanceSterile Filtered colorless solution
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | The H1N1 A/Solomon Islands/03/06 solution contains 0.1M NaCl, 10mM Tris-HCl, 1mM EDTA pH-8 (STE), 0.1% sodium azide (NaN3) and 0.005% thimerosal. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Description | Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Solomon Islands/03/06. The Influenza Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient. |
| Protein Background | H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flu strain, mild human flu strains, endemic pig strains, and various strains found in birds. The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function. |
| Expression host | Baculovirus Insect Cells. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | A/Solomon Islands/03/06 although stable 4°C for 4 weeks, should be stored below -18°C. Please prevent freeze-thaw cycles. |
| References | Title: Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccinePublication: The Journal of clinical investigation 120.5 (2010): 1663.Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860935/ |
| Antibody Properties | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Protein content | By direct ELISA (against recombinant gp120), 1:20,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Application And Suggested Dilutions | This is a binding and neutralizing antibody. Neutralization was tested in tissue culture. |
| Product Description | Immunoreactive with all sera of HIV-1 and HIV-type O infected individuals and with 60-80% of HIV-2 infected individuals. |