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Hydroxyacyl-Coenzyme A Dehydrogenase Human Recombinant ( HADH Human )
DescriptionHADH Human Recombinant fused to 21 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 323 amino acids (13-314 a.a.) and having a molecular mass of 35.1 kDa. The HADH is purified
Data sheet
| Formulation | The HADH solution contains 20mM Tris-HCl pH-8, 0.1M NaCl and 20% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | HADH Human Recombinant fused to a 21 amino acids His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 323 amino acids (13-314 a.a.) and having a molecular mass of 35.1 kDa. The HADH is purified by proprietary chromatographic techniques. |
| Protein Background | HADH is part of the 3-hydroxyacyl-CoA dehydrogenase enzyme family. HADH is involved in mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. HADH enzymatic activity is at its peak with medium-chain-length fatty acids. Mutations in HADH cause familial hyperinsulinemic hypoglycemia. HADH participates in fatty acid oxidation, where some enzymes work in a step-wise fashion to break metabolize fats and convert them to energy. |
| Expression host | Escherichia Coli. |
| Synonyms | EC 1.1.1.35, HAD, HADH1, HHF4, MSCHAD, SCHAD, Hydroxyacyl-coenzyme A dehydrogenase, HCDH, Short-chain 3-hydroxyacyl-CoA dehydrogenase, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, HADH, HADHSC, MGC8392. |
| Reagent Appearance | Sterile Filtered clear colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSSSSTASAS AKKIIVKHVT VIGGGLMGAG IAQVAAATGH TVVLVDQTED ILAKSKKGIE ESLRKVAKKK FAENPKAGDE FVEKTLSTIA TSTDAASVVH STDLVVEAIV ENLKVKNELF KRLDKFAAEH TIFASNTSSL QITSIANATT RQDRFAGLHF FNPVPVMKLV EVIKTPMTSQ KTFESLVDFS KALGKHPVSC KDTPGFIVNR LLVPYLMEAI RLYERGDASK EDIDTAMKLG AGYPMGPFEL LDYVGLDTTK FIVDGWHEMD AENPLHQPSP SLNKLVAENK FGKKTGEGFY KYK. |
| Quality Control Test | slyn tvatlycvhq rieikdtkea ldkikeeqnk skkkaqqaaa dtghssqvsq nypivqniqg qmvhqaispr tlnawvkvve ekafspevip mfsalsegat pqdlntmlnt vgghqaamqm lketineeaa ewdrvhpvha gpiapgqmre prgsdiagtt stlqeqigwm tnnppipvge iykrwiilgl nkivrmyspt sildirqgpk epfrdyvdrf yktlraeqas qevknwmtet llvqnanpdc ktilkalgpa atleemmtac qgvggpghka rvlaeamsqv tnsatimmqr gnfrnqrkiv kcfncgkegh iarncraprk kgcwkcgkeg hqmkdcterq anflgk. |
| Product Description | Immunoreactive with all sera of HIV-1 infected individuals. |