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Hepatitis C Virus Core Genotype-3a Recombinant ( HCV Core Genotype-3a )
DescriptionThe E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. The protein is fused to a GST tag at N-terminus.Purification MethodHCV Core Genotype-3a protein was purified by
Data sheet
| Storage | HCV Core Genotype-3aalthough stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Application | Reduces non-specific binding Improves specificity Consistent Immunoglobin Free Protease free Outperforms BSA and pHSA Enhances stability of antibodies and analytes |
| Formulation | (1mg/ml) 1.5M urea, 25mM Tris-HCl, pH 8.0, 0.2% Triton-X and 50% glycerol. |
| Purity | HCV Core Genotype-3a protein is >95% pure as determined by 10% PAGE (coomassie staining). |
| Applications | HCV Core Genotype-3a antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems. |
| Description | The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. The protein is fused to a GST tag at N-terminus. |
| Protein Background | HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). |
| Expression host | Escherichia Coli. |
| Purification Method | HCV Core Genotype-3a protein was purified by proprietary chromatographic technique. |
| Introdcution | Recombinant Albumin can be used as a media culture supplement at concentrations up to 5 grams per liter. Gradual adaptation of cell lines over several passages to a concentration of 0.5gr to 2gr per liter. |