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Hepatitis C Virus NS3 Genotype-1b Recombinant ( HCV NS3 Genotype-1b )
DescriptionThe E.coli derived 26.2 kDa recombinant protein contains the HCV NS3 c33c immunodominant regions. The 252 amino acid protein is fused to 6xHis tag.Purification MethodPurified by proprietary chromatographic technique.PurityHCV NS3
Data sheet
| Storage | HCV NS3 Genotype-1b although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 50mM NaPO4 pH 8.3 and 10mM DTT. |
| Purity | HCV NS3 Genotype-1b protein is >95% pure as determined by 10% PAGE (coomassie staining). |
| Applications | HCV NS3 Genotype-1b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems. |
| Description | The E.coli derived 26.2 kDa recombinant protein contains the HCV NS3 c33c immunodominant regions. The 252 amino acid protein is fused to 6xHis tag. |
| Protein Background | HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). |
| Purification Method | Purified by proprietary chromatographic technique. |
| Reference | Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP20. |