View larger
Hepatitis C Virus NS5 Genotype-1a Recombinant ( HCV NS5 Genotype-1a )
DescriptionThe E.coli derived 38 kDa recombinant protein contains the HCV NS5 Genotype1a immunodominant regions, amino acids 2212-2313.Purification MethodHCV NS5 Genotype-1a protein was purified by proprietary chromatographic technique.PurityHCV NS5
Data sheet
| Storage | HCV NS5 Genotype-1a although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 50mM Tris, pH 8 & 5mM EDTA. |
| Purity | HCV NS5 Genotype-1a protein is >95% pure as determined by 10% PAGE (coomassie staining). |
| Applications | HCV NS5 Genotype-1a antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems. |
| Description | The E.coli derived recombinant protein contains the HCV NS5 Genotype1a immunodominant regions, amino acids 2322-2423 having a total Mw of 38.34kD which includes a 26Kda GST tag. |
| Protein Background | HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). |
| Purification Method | HCV NS5 Genotype-1a protein was purified by proprietary chromatographic technique. |