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Hepatitis A Virus P3C Recombinant ( HAV P3C )
DescriptionThe E.Coli derived 40 kDa recombinant protein contains the P3C immunodominant regions, amino acids 1643-1743.Purification MethodHAV P3C protein was purified by proprietary chromatographic technique.PurityHAV P3C protein is >90% pure as
Data sheet
| Storage | HAV P3C protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 10mM CBB, pH9.6, 0.1% SDS and 50% glycerol. |
| Purity | HAV P3C protein is >90% pure as determined by 10% PAGE(coomassie staining). |
| Applications | HAV P3C antigen is suitable for ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems. |
| Description | The E.Coli derived 40 kDa recombinant protein contains the P3C immunodominant regions, amino acids 1643-1743. |
| Protein Background | Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins. |
| Expression host | Escherichia Coli. |
| Purification Method | HAV P3C protein was purified by proprietary chromatographic technique. |
| Product Description | Immunoreactive with HIV-I gp41. Generates a Strong positive control spot on HIVSav 1+2. Generates 1 OD (410nm) at a dilution of 1: 250 on Rec HIV-1 trans membrane protein in ELISA. |