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Interleukin-17E Human Recombinant ( IL 17E Human )
DescriptionInterleukin-17E Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of 33.7 kDa. The IL-25 Human is purified by proprietary chromatographic
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | Lyophilized from a concentrated (1mg/ml) solution containing no additives. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin Human IL25 in sterile 10mM HCl not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Interleukin-17E Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of 33.7 kDa. The IL-25 Human is purified by proprietary chromatographic techniques. |
| Protein Background | IL-25 also called IL-17E cytokine has a sequence similarity with IL17. IL-17E indluces NF-kappaB activation, and stimulates the production of IL-8. IL17E and IL17B are ligands for the cytokine receptor IL17BR. IL-25 is a proinflammatory cytokine favoring Th2-type immune response. The upregulation of costimulation-induced IL-17E receptors and release of cytokines and chemokines from IL-17E treated costimulated Th cells are differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. Blocking Iinterleukin-25 prevents airway hyperresponsiveness, a critical feature of clinical asthma. IL25 produced by innate effector eosinophils and basophils increase the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells. Over expression of IL-25 up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. IL-25 contributes to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts which supports the fact that IL-17E is an important factor in asthma pathophysiology. IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesnt induce allergic inflammation in vivo. |
| Expression host | Escherichia Coli. |
| Synonyms | IL-25, IL-17E, IL17E, IL25, Interleukin-25, Interleukin-17E. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Interleukin Human IL17E although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human IL-25 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG. |
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Protein content | Not tested by ELISA. 1:1,000 dilution will block T cell proliferation induced by specific Ag or concanavaline A. |