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Interleukin-3 Human Recombinant ( IL 3 Human )
DescriptionInterleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic
Data sheet
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques. |
| Protein Background | IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. |
| Expression host | Escherichia Coli. |
| Synonyms | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is |
| References | 1.Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .Link:http://www.fasebj.org/content/24/8/2869.full2.Title:A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation.Publication:Article first published online: 24 AUG 2006 DOI:10.1634/stemcells.2005-0657 Copyright © 2006 AlphaMed PressLink:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2005-0657/full3.Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent PathwayPublications: PloS one 8.12 (2013): e82867Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082867#pone-0082867-g0054. Title: Novel markers of normal and neoplastic human plasmacytoid dendritic cellsPublication: Blood 111.7 (2008): 3778-3792.Link: http://www.bloodjournal.org/content/111/7/3778 |