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Interleukin-4 Human Recombinant ( IL 4 Human )
DescriptionInterleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The IL-4 is purified by proprietary chromatographic
Data sheet
| Formulation | Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15kDa. The IL-4 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Synonyms | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is |
| References | 1. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation1Publication:The Journal of Immunology, 2006, 177: 6695-6704.Copyright © 2006 by The American Association of Immunologists, Inc.Link:http://www.jimmunol.org/content/177/10/6695.full2.Title:CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapsePublication:Published online before print June 27, 2005, doi: 10.1073/pnas.0501050102 PNAS July 5, 2005 vol. 102 no. 27 9583-9588 Link:http://www.pnas.org/content/102/27/9583.full3.Title:A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic DiseasePublication:doi: 10.4049/?jimmunol.0804125 The Journal of Immunology May 15, 2009 vol. 182 no. 10 6011-6021 Link:http://www.jimmunol.org/content/182/10/6011.full4.Title:Defective IL-10 production in severe phenotypes of Crohns diseasePublication:Published online before print February 23, 2009, doi: 10.1189/jlb.1108698 May 2009 Journal of Leukocyte Biology vol. 85 no. 5 896-903 Link:http://www.jleukbio.org/content/85/5/896.full5.Title:Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells .Publication:Published online before print October 19, 2010, doi: 10.1189/jlb.1009696 January 2011 Journal of Leukocyte Biology vol. 89 no. 1 127-136 .Link:http://www.jleukbio.org/content/89/1/127.full6.Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation/Publication:Link:http://journal.9med.net/qikan/article.php?id=3677937.Title:Airway Epithelial Cells Regulate the Functional Phenotype of Locally Differentiating Dendritic Cells: Implications for the Pathogenesis of Infectious and Allergic Airway Disease.Publication: The Journal of Immunology January 1, 2009 vol. 182 no. 1 72-83 Link:http://www.jimmunol.org/content/182/1/72.full8. Title: Influence of heat stress on human monocyte-derived dendritic cell functions with immunotherapeutic potential for antitumor vaccinesPublications: Journal of l |