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Interleukin-23 Human Recombinant ( IL 23 Human )
DescriptionIL-23 Human Recombinant produced in HEK 293 cells is a disulfide-linked heterodimer, containing one polypeptide chain p19 subunit containing 169 amino acids and one polypeptide chain IL-12B p40 subunit containing 306 amino acids, with the
Data sheet
| Formulation | IL-23 was lyophilized from a concentrated (480ug/ml) sterile solution containing 1x PBS. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-23 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Description | IL-23 Human Recombinant produced in HEK cells is a 55kDa heterodimeric protein composed of 2 disulfide-linked subunits - 19kDa (p19) and 43 kDa (p40). |
| Protein Background | IL23 is composed of a subunit of the heterodimeric cytokine IL23 and the p40 subunit of interleukin 12 (IL12B). Interleukin-23 (IL-23) belongs to the IL-12 family and is produced by antigen presenting cells. IL-23 using IL12RB1 and IL-23R (specific for IL-23) can activate STAT and NF-kB pathways and stimulate the production of interferon-gamma. ). IL-23 is known to take a vital part in the inflammation process and is associated with auto immune diseases. However, unlike IL12, which acts primarily on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. |
| Expression host | HEK. |
| Synonyms | Interleukin 23 alpha subunit p19, Interleukin-12 subunit beta p40, SGRF, IL23P19, IL-23-A, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, interleukin 12B (natural killer cell stimulatory factor 2 cytotoxic lymphocyte maturation factor 2 p40), NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF2, CLMF p40. |
| Reagent Appearance | IL-23 if supplied as a sterile filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Interleukin-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-23 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Biological Activity | The activity, as determined by the dose dependent secretion of h-IFN-g from human PBMC was found to be 2 to 20ng/ml ED50. |