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Influenza-B Virus Florida 07/04 ( Influenza-B Florida )
DescriptionAllantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain B/Florida/07/04. The Influenza B Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.InactivationThimerosal and beta
Data sheet
| Formulation | The B/Florida/07/04 solution contains STE, 0.09% sodium azide (NaN3) and 0.005% thimerosal. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Inactivation | Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Horse as a Reference Standard. |
| Description | Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain B/Florida/07/04. The Influenza B Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient. |
| Protein Background | Influenza-B virus is a genus in the virusfamily Orthomyxoviridae. The only species in this genus is called "Influenza B virus". Influenza B virus only infects humansand seals. This limited host range is apparently in contrast with those caused by the similar Influenza virus A as both mutate by both genetic drift and reassortment. Influenza-B virus evolves slower than A viruses and faster than C viruses. Influenza-B virus mutates at a rate 2-3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible. The Influenza B virus capsidis enveloped while its virionconsists of a matrix protein + envelope + nucleoprotein complex + nucleocapsid, and a polymerasecomplex. Influenza B is sometimes spherical and sometimes filamentous. Its 500 or so surface projections are made of hemagglutinin and neuraminidase.The Influenza B virus is 14648 nucleotideslong and consists of eight segments of linear negative-sense, single-stranded RNA. The multipartite genome is encapsidated, each segment in a separate nucleocapsid, and the nucleocapsids are surrounded by one envelope. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | B/Florida/07/04 although stable 4°C for 4 weeks, should be stored below -18°C. Please prevent freeze-thaw cycles. |