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Influenza-B Virus Ohio 01/05 Recombinant ( Influenza-B Ohio )

DescriptionRecombinant Full-Length B/Ohio/01/05 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells.SourceBaculovirus Insect Cells.Physical AppearanceSterile Filtered colorless solution.FormulationThe Recombinant

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Data sheet

Formulation The Recombinant B/Ohio/01/05 solution contains 30mM Sodium phosphate, pH 7.4 and 50mM NaCl.
Purity Greater than 90.0% as determined by SDS-PAGE.
Inactivation Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Ovine as a Reference Standard.
Description Recombinant Full-Length B/Ohio/01/05 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells.
Protein Background Influenza-B virus is a genus in the virusfamily Orthomyxoviridae. The only species in this genus is called "Influenza B virus". Influenza B virus only infects humansand seals. This limited host range is apparently in contrast with those caused by the similar Influenza virus Aas both mutate by both genetic drift and reassortment. Influenza-B virus evolves slower than A viruses and faster than C viruses. Influenza-B virus mutates at a rate 2-3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible. The Influenza B virus capsidis enveloped while its virionconsists of a matrix protein + envelope + nucleoprotein complex + nucleocapsid, and a polymerasecomplex. Influenza B is sometimes spherical and sometimes filamentous. Its 500 or so surface projections are made of hemagglutinin and neuraminidase.The Influenza B virus is 14648 nucleotideslong and consists of eight segments of linear negative-sense, single-stranded RNA. The multipartite genome is encapsidated, each segment in a separate nucleocapsid, and the nucleocapsids are surrounded by one envelope.
Expression host Baculovirus Insect Cells.
Reagent Appearance Sterile Filtered colorless solution.
Stability B/Ohio/01/05 Recombinant should be stored at 4°C.

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