Cart
0
Product
Products
(empty)
No products
To be determined
Shipping
$0.00
Total
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart.
There is 1 item in your cart.
Total products
Total shipping
To be determined
Total
View larger
Lactate Dehydrogenase B Recombinant ( LDHB )
DescriptionThe DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.SourceEscherichia Coli.Physical AppearanceSterile lyophilized powder.FormulationThe protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.SolubilityIt
Data sheet
| Formulation | The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate. |
| Solubility | It is recommended to reconstitute the lyophilized LDH in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. |
| Protein Background | Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. |
| Expression host | Escherichia Coli. |
| Synonyms | Lactate Dehydrogenase, LDH. |
| Reagent Appearance | Sterile lyophilized powder. |
| Stability | Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Unit Definition | One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity. |
| Biological Activity | The specific activity was found to be 258 U/mg protein. |
| References | Title: UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE) Binds to Alpha-Actinin 1: Novel Pathways in Skeletal Muscle? Publications: PLoS One 3.6 (2008): e2477. Link: http://journals.plos.org/plosone/articleid=10.1371/journal.pone.0002477#s1 |