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Plasmodium Falciparum HSP70 Recombinant ( PfHSP70 )
DescriptionThe E.Coli derived recombinant Plasmodium falciparum contains the Plasmodium falciparum HSP70 protein epitopes 33-114 amino acids.MethodPurified by proprietary chromatographic technique.PurityProtein is >95% pure as determined by 10% PAGE
Data sheet
| Storage | PfHSP70 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 150mM Imidazole, pH 8.0, 150mM NaCl, 150mM Sodium Phosphate and 50% glycerol. |
| Purity | Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
| Applications | Use as an antigen in ELISA and Western Blots. |
| Inactivation | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard. |
| Description | The E.Coli derived recombinant Plasmodium falciparum contains the Plasmodium falciparum HSP70 protein epitopes 33-114 amino acids. |
| Protein Background | Plasmodium falciparum heat shock proteins (PfHSPs) contribute to immunopathological alterations in infected hosts PfHSP70s may have a significant function during parasite adaptation in its environments. They have also been a focus of considerable attention due to their immunodominant antigenic nature and their properties as mediators of protective immunity. |
| Reagent Appearance | Sterile Filtered clear solution. |