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Phosphogluconate Dehydrogenase Human Recombinant ( PGD Human )
DescriptionPGD Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids (1-483 a.a.) and having a molecular mass of 55.3 kDa. The PGD is fused to a 20 amino acid His Tag at N-terminus and
Data sheet
| Formulation | The PGD solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | PGD Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids (1-483 a.a.) and having a molecular mass of 55.3 kDa. The PGD is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background | 6PGD is the second dehydrogenase in the pentose phosphate shunt. Pentose is neccesary for nucleic acid biosynthesis. The pentose phosphate cycle is a major source of NADPH 6PGD deficiency is usually asymptomatic, and the inheritance of this disorder is autosomal dominant. PGD deficiency raises the erythrocyte pyruvate kinase levels of activity and decreases glutathione synthetase, resulting in hemolysis. |
| Expression host | Escherichia Coli. |
| Synonyms | EC 1.1.1.44, 6PGD, PGDH, 6-phosphogluconate dehydrogenase decarboxylating, PGD. |
| Reagent Appearance | Sterile Filtered clear colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA. |