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Phosphoinositide 3-kinase p110d/p85a Human Recombinant ( PI3Kd Human )
DescriptionThe class IA PI3K? is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3K? in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and
Data sheet
| Formulation | PI3Kd solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol. |
| Purity | Greater than 95.0% as determined by SDS Page. |
| Description | The class IA PI3Kd is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kd in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3Kd in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the proteins application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110d chain is 120.2 kDa (without Tag) and the p85a chain 83.5 kDa. |
| Expression host | Sf9 insect cells. |
| Synonyms | Phosphoinositide 3-kinase p110d/p85a, PI3Kd. |
| Reagent Appearance | Sterile filtered liquid formualtion. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Unit Definition | 1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute. |
| Activity | 3,000 U/mg. |