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Proteinase-3 Human ( PRTN3 Human )
DescriptionPRTN3 is a natural antigen having a molecular mass of 25kDa to 32kDa due to glycosylation isoforms. PRTN3 is isolated from human peripheral blood leukocytes.SourceNative.FormulationPRTN3 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl,
Data sheet
| Formulation | PRTN3 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. |
| Purity | Greater than 80% in the sum of different glycosylation isoforms according to following section as determined by SDS-PAGE and capillary electrophoresis. |
| Applications | Western blot with rabbit anti-PR3 antisera and mouse anti-PR3 monoclonal antibodies. |
| Description | PRTN3 is a natural antigen having a molecular mass of 25kDa to 32kDa due to glycosylation isoforms. PRTN3 is isolated from human peripheral blood leukocytes. |
| Protein Background | PRTN3 is a polymorphonuclear leukocyte serine protease which degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema once managed by tracheal insufflation to hamsters. |
| Expression host | Native. |
| Synonyms | AGP7, P29, PR-3, ACPA, C-ANCA, MBT, MBN, Leukocyte proteinase 3, Neutrophil proteinase 4, Wegener granulomatosis autoantigen, Azurophil granule protein 7, myeloblastin, Serine proteinase neutrophil. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Coating concentration | 0.5-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |