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Selenocysteinyl-tRNA ( Sec ) synthase Human Recombinant ( SEPSECS Human )
DescriptionSEPSECS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 521 amino acids (1-501a.a.) and having a molecular mass of 57.9kDa.SEPSECS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary
Data sheet
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Formulation | The SEPSECS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | SEPSECS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 521 amino acids (1-501a.a.) and having a molecular mass of 57.9kDa.SEPSECS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | SEPSECS catalyzes the last step of sec synthesis by way of converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Furthermore, SEPSECS protein is considered a specific marker of autoimmune hepatitis. |
| Expression host | Escherichia Coli. |
| Synonyms | SLA, SLA/LP autoantigen, SLA-p35, Selenocysteinyl-tRNA(Sec) synthase, Selenocysteine synthase, Liver-pancreas antigen, Soluble liver antigen, Sec synthase, UGA suppressor tRNA-associated protein. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S. |