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Human Thrombin ( Thrombin Human )
DescriptionThrombin was purified from human plasma.SourceHuman Plasma.Physical AppearanceSterile filtered liquid formulation.FormulationThe Factor-IIa contains 50mM sodium Citrate, 0.2M NaCl & 0.1% PEG-8000 pH 6.5.StabilityHuman Thrombin although
Data sheet
| Formulation | The Thrombin solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Purity | Greater than 80.0% as determined by SDS-PAGE. |
| Description | Thrombin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (328-622) and having a molecular mass of 33.9 kDa. |
| Protein Background | Coagulation factor II (F2) is proteolytically cleaved to form thrombin in the 1st step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also has a role in preserving vascular integrity during development and postnatal life. F2 gene mutations lead to various forms of thrombosis and dysprothrombinemia. |
| Expression host | Escherichia Coli. |
| Synonyms | Coagulation Factor II (Thrombin), Prepro-Coagulation Factor II, EC 3.4.21.5, RPRGL2, THPH1, Coagulation Factor II, Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, PT, F2. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE. |
| Biological Activity | The specific activity was found to be 3026 NIH Units/mg. |