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Tumor Necrosis Factor-Alpha Human Recombinant ( TNF a Human )
DescriptionTumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of
Data sheet
| Formulation | 1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl. |
| Solubility | It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa. The TNF-alpha is purified by standard chromatographic techniques. |
| Protein Background | Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. |
| Expression host | Escherichia Coli. |
| Synonyms | TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL. |
| Biological Activity | The Specific Activity is >5.0107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D. |
| References | 1.Title: Radiation-triggered Tumor Necrosis Factor (TNF) ?-NF?B Cross-signaling Favors Survival Advantage in Human Neuroblastoma Cells*Publication:First Published on April 28, 2011, doi: 10.1074/jbc.M110.193755 June 17, 2011 The Journal of Biological Chemistry, 286, 21588-21600. Link:http://www.jbc.org/content/286/24/215882.Title:Bidirectional Concentration-Dependent Effects of Tumor Necrosis Factor Alpha in Shigella dysenteriae-Related Seizures.Publication:INFECTION AND IMMUNITY, Apr. 2003, p. 22882291 Vol. 71, No. 40019-9567/03/$08.000 DOI: 10.1128/IAI.71.4.22882291.2003Copyright © 2003, American Society for Microbiology. All Rights Reserved.Link:http://iai.asm.org/content/71/4/2288.full.pdf 3.Title:Common Inhibitory Serine Sites Phosphorylated by IRS-1 Kinases, Triggered by Insulin and Inducers of Insulin Resistance.Publication:First Published on April 19, 2007, doi: 10.1074/jbc.M610949200 June 22, 2007 The Journal of Biological Chemistry, 282, 18018-18027. Link:http://www.jbc.org/content/282/25/18018.full4.Title:Antioxidative function of L-FABP in L-FABP stably transfected Chang liver cells.Publication:Article first published online: 20 SEP 2005 DOI:10.1002/hep.20857 Copyright © 2005 American Association for the Study of Liver Diseases.Link:http://onlinelibrary.wiley.com/doi/10.1002/hep.20857/full5.Title:Interferon-? induces apoptosis in human SH-SY5Y neuroblastoma cells through activation of JAKSTAT signaling and down-regulation of PI3K/Akt pathwayPublication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046.x © 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2010.07046.x/full6.Title:Common Inhibitory Serine Sites Phosphorylated by IRS-1 Kinases, Triggered by Insulin and Inducers of Insulin Resistance.Link:http://journal.9med.net/qikan/article.php?id=4087627.Title:The human respiratory syncytial virus non-structural protein 1 regulates type I and type II i |