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Urokinase Human ( Urokinase Human )
DescriptionUrokinase is a two-chain glycoprotein containing 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000 Dalton.SourceHuman urine.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationThe
Data sheet
| Formulation | The Urokinase was lyophilized from a concentrated (1mg/ml) solution containing phosphate buffer. |
| Solubility | It is recommended to reconstitute the lyophilized Urokinase in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 94.0% as determined by SDS-PAGE. |
| Description | Urokinase is a two-chain glycoprotein containing 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000 Dalton. |
| Protein Background | Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. |
| Expression host | Human urine. |
| Synonyms | Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Urokinase should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Amino acid sequence | SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA LLEHHHHHH. |
| Specific Activity | 174,827IU/mg. |
| References | 1. Title:Transient ?-catenin stabilization modifies lineage output from human thymic CD34+CD1a progenitors. Publication:Published online before print December 1, 2009, doi: 10.1189/jlb.0509344 March 2010 Journal of Leukocyte Biology vol. 87 no. 3 405-414 .Link:http://www.jleukbio.org/content/87/3/405.full2. Title: The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysinPublications: Biochem. J 410 (2008): 157-165.Link: http://www.biochemj.org/bj/410/bj4100157.htm3. 2.Title:Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.Publication:Biochem. J 428 (2010): 473-482.Link:http://www.biochemj.org/bj/428/0473/4280473.pdf |