Porcine IL-1 beta ELISA kit

Catalog # BG-POR50010

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$715.00
Quantity

Assay Range

62.5-4,000 pg/mL

Sensitivity

10.0 pg/mL

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Sample Type

Cell culture supernatants.

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Porcine IL-1b standard: 2 vials

 2. One 96-well plate coated with Porcine IL-1b  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 1 vial

 5.Streptavidin-HRP: 1 vial

 6. Antibody diluent buffer: 12 mL x1  

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing reagent:  10 mlx1

 9. Stop solution:  10 mL x1

10. Washing solution (20x):  25 mL x1

 

Background     

The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1α, IL-1β, IL-1ra, IL-18, IL-33 and IL-1F5-10. In response to inflammatory agents, infections, or microbial endotoxins, IL-1 is significantly produced by macrophages and various other cell types. IL-1α is mainly expressed and secreted by activated macrophages, neutrophils, epithelial cells, and endothelial cells. It plays a key role in regulating the immune responses. IL-1α is approximately 25% identical to IL-1β. Both IL-1α and IL-1β are synthesized as 31 kDa precursors that can be further cleaved into mature proteins of approximately 17.5 kDa. Cleavage of the IL-1β precursor by Caspase-1/ICE is a key step in the inflammatory response. The precursor form of IL-1β, unlike the IL-1αprecursor, exhibits little or no biological activity in comparison to its mature form.

 

IL-1α and IL-1β bind to transmembrane type I receptor (IL-1 RI) associated with IL-1 RAcP and type II receptor (IL-1 RII) to activate the downstream signaling pathways. IL-1ra is a secreted protein and functions as a competitive inhibitor of IL-1. IL-1 RII itself does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function. IL-1β plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis.

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