More info
Size | 96T |
Specificity | No cross-reaction with other related substances detected |
Storage | Store at 2 - 8ºC. Keep detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA/Qualitative Detection |
Sample Volume | 100 µL final volume |
Sample Type | Serum or plasma |
Detection Method | Chromogenic |
Kit Components
1. One 96-well plate pre-coated purified NDV antigen
2. Negative Control: 1 vial
3. Positive Control: 1 vial
4. Diluent buffer: 30 ml
5. NDV Ab -HRP Conjugate: 1 vial
6. Chromogenic solution A 6 mL x 1
7. Chromogenic solution B 6 mL x 1
8. Stop solution:6 mL x1
9. Washing solution (20x): 1 vial
Assay principle
The NDV ELISA kit applies a competitive enzyme immunoassay technique. The test sample and HRP conjugated NDV antibody are added into the microplate wells preâ€coated with purified NDV antigen and incubated appropriately. The NDV antibody in the sample, if presents, and the HRP conjugate will compete for binding to NDV antigen immobilized on the plate wells. The wells are then decanted and washed with wash buffer. And then, the wells are incubated with a substrate (TMB) for HRP. The product of the enzymeâ€substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the amount of NDV Ab, as NDV Ab from sample and HRP conjugated NDV Ab compete for the NDV antigen binding site.