More info
Assay Range | 31.2-1,000 pg/mL |
Sensitivity | 10 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | Cell lysates |
Detection Method | Chromogenic |
Kit Components
1. Recombinant human IQGAP3 standard: 6 vials
2. One 96-well plate coated with human IQGAP3 Ab
3. Diluent buffer: 6 mL - 1
4. Detection antibody: 1 vial
5. Chromogenic solution A: 6 mlx1
6. Chromogenic solution B: 6 mlx1
7. Stop solution: 6 mL x1
8. Washing solution (20x): 25 mL x1
Background
IQGAP3 (IQ motif containing GTPase activating protein 3) is a member of the IQGAP family including IQGAP1, IQGAP2 and IQGAP3. IQGAP3 is a 1,631 amino acid protein consisting of one Ras-GAP domain, a CH (calponin-homology) domain, four IQ domains. IQGAP3 is highly expressed in axons of hippocampal neurons. IQGAP3 functions as an effector of Cdc42 and Rac 1, linking their activation to the cytoskeleton during neuronal morphogenesis. IQGAP3 is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma.