More info
Assay range | 0.156-40 ng/ml |
Sensitivity | 0.15 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Plasma, serum, and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human SCF standard: 1 vial
2. One 96-well plate coated with Human SCF Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human SCF Ab (80x): 100 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Stem cell factor (SCF), also known as c-Kit receptor ligand (KL), steel factor, or mast cell growth factor (MGF), is a transmembrane protein composed of a 25 amino acid (aa) residue signal peptide, a 189 aa extracellular domain, a 23 aa transmembrane span, and a 36 aa cytoplasmic segment. Two alternatively spliced forms of SCF have been found in human and mouse. Both the larger 45 kDa form (KL-1) and the smaller 32 kDa form (KL-2) are cleaved to produce soluble factors. Cleavage of KL-1 gives rise to a 31 kDa soluble form, while cleavage of KL-2 generates a 23 kDa soluble molecule. SCF has been identified in various cell types, such as fibroblasts, liver cells, Sertoli cells, endothelial cells, neurons, macrophages, oocytes, and Schwann cells. Human soluble SCF is highly homologous to Mouse or rat soluble SCF. Although rat and mouse SCF are active on human cells, human SCF is much less active on mouse or rat cells. It has been shown that both the soluble and the transmembrane forms of SCF have growth factor activities, and the transmembrane form play an important role in mediating cell-cell adhesion and interaction. In addition, SCF has exhibited multiple bioactivities in hematopoiesis, reproduction, melanogenesis, proliferation and differentiation of mast cells, as well as tumor progression. Clinically, increased expression of SCF and its receptor c-Kit were found in asthma patients. During chronic stroke, SCF in combination with granulocyte-colony stimulating factor (G-CSF) treatment can enhance repair of brain damage.