More info
Assay range | 1.25-80 ng/ml |
Sensitivity | 1.2 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, saliva, milk, CSF, and cell culture supernantants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Prekallikrein standard: 1 vial
2. One 96-well plate coated with Human Prekallikrein Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Prekallikrein Ab (50x): 140µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Human Plasma Kallikrein is a member of the serine protease family. It is composed of a 19 amino acid (aa) signal peptide and a 619 aa propeptide. Upon activation, the propeptide is converted to a heavy chain and a light chain (containing the catalytic domain) linked by disulfide bonds. It is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. Primarily, it cleaves HMW kininogen to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Therefore, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation. Kallikrein-deficient patients have prolonged activated partial thromboplastin time without having any bleeding disorder.