More info
Assay range | 6.25-400 µg/ml |
Sensitivity | 6.0 µg/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Competitive ELISA |
Sample Volume | 25 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, milk and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human HDL standard: 1 vial
2. One 96-well plate coated with Human HDL Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human HDL: 1 ival
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Lipoproteins are complex particles composed of multiple proteins which transport all fat molecules (lipids) in the body. High-density lipoprotein (HDL) is the smallest and dentest of the five major groups of lipoproteins. It contains 70% of apolipoprotein A-I, 20% of apolipoprotein A-II, phospholipids, and free cholesterol. Unlike the larger lipoprotein particles which deliver fat molecules to cells, HDL particles remove fat molecules from cells which want to export fat molecules. It exhibits various activities, such as cellular cholesterol efflux capacity, anti-oxidative, anti-inflammatory, antiapoptotic, vasodilatory, antithrombotic, and anti-infectious activities. Low plasma HDL cholesterol is an independent risk factor for the development of premature atherosclerosis. High levels of HDL (over 60 mg/dL) have protective value against cardiovascular diseases such as ischemic stroke and myocardial infarction.