More info
Assay range | 3.12-200 ng/ml |
Sensitivity | 3.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Urine, saliva, milk and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human AGP standard: 1 vial
2. One 96-well plate coated withHuman AGP Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human AGP Ab (30x): 270 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Alpha-1-acid glycoprotein (AGP), also known as orosomucoid (ORM), α1AGp or AAG, is a 183 amino acid highly glycosylated (45% ) protein with a moleculr weight of 41 KD. It is an acute-phase plasma glycoprotein synthesized in the liver and secreted into the plasma. In response to acute infections or inflammation, the plasma concentration of AGP increases considerably. The elevated serum level of AGP is associated with an increased risk of cardiovascular disease. It is reported that urinary AGP excretion rate predicts cardiovascular mortality in patients with Type II diabetes. AGP can be used as a marker for acute inflammation, chronic alcohol drinking, chronic kidney disease and asthma.