More info
Assay range | 1.25-40 ng/ml |
Sensitivity | 1.2 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | platelets, platelet-rich plasma, and cell culture lysate. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human GPIIb/IIIa standard: 1 vial
2. One 96-well plate coated withHuman GPIIb/IIIa Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human GPIIb/IIIa Ab (100x): 80 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa), also known as integrin αIIbβ3, is a member of the integrin family of cell membrane receptors that play key roles in thrombus formation, platelet aggregation, embryogenesis, and intercellular adhesion. Each integrin receptor complex consists of a heavy (alpha) and a light (beta) chain associated as a calcium-dependent heterodimer. Platelet activation leads to a conformational change in platelet gpIIb/IIIa receptors that induces binding to fibrinogen. Defects in glycoprotein IIb/IIIa cause Glanzmann's thrombasthenia. Glycoprotein IIb/IIIa inhibitors can prevent blood clots and decrease the risk of heart attack or stroke.