More info
Assay range | 625-40,000 pg/mL |
Sensitivity | 600 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma,body fluids, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoH standard: 1 vial
2. One 96-well plate coated with Human ApoH Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoH Ab (100x): 80 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein H (ApoH), also known as β2-Glycoprotein I (β2GPI), is a glycosylated protein of the complement control superfamily. Mature human ApoH is characteristic of four tandem Sushi/SCR repeats and one Sushilike repeat. Mature human ApoH shares 76% and 82% aa sequence identity with mouse and rat ApoH, respectively. It circulates as a component of lipoprotein particles and as a lipid-free serum protein. Circulating levels of ApoH are postively correlated with triglyceriderich lipoprotein (VLDL) components in type II diabetes. ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C. ApoH can be cleaved by Plasmin at Lys317 - Thr318, which reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen. Production of ApoH antibodies is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages.