More info
Assay range | 62.5-4,000 ng/ml |
Sensitivity | 60.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoC-II standard: 1 vial
2. One 96-well plate coated with Human ApoC-II Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoC-II Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein C2 (ApoC2/II) is a 8-9 kDa, secreted protein of the Apolipoprotein C2 family. It is produced by hepatocytes and is a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid type fibrils. The full-length human ApoC2 is an 101 amino acid (aa) precursor protein composed of a 22 amino acid (aa) signal sequence, a 79 aa ProApoC2 containing a lipid binding region (aa 43-51) and an enzyme interaction site (aa 55-78). More than 90% of circulating ApoC2 exists in the form of ProApoC2. Human ProApoC2 is 59% aa identical to mouse counterpart. It is reported that apoC-II aggregates are present in human atherosclerotic plaques and its fibrils initiate macrophage inflammatory responses. Deficency of either ApoC-II or lipoprotein lipase results in hypertriglyceridemia.