More info
Assay range | 15.6-1,000 ng/ml |
Sensitivity | 15.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoC-I standard: 1 vial
2. One 96-well plate coated with Human ApoC-I Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoC-I Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein C-I (ApoC-I) is a member of the apolipoprotein C family. Synthesized as an 83 amino acid (aa) precursor protein with a molecular weight of 6.6 kDa, ApoC-I is processed to a 57 aa single chain mature protein. ApoC-I is expressed primarily in the liver and activated when monocytes differentiate into macrophages. It circulates in plasma and is a component of VLDL, IDL, and HDL. ApoC-I plays important modulatory roles in lipoprotein metabolism. It inhibits lipoprotein binding to the LDL receptor, LDL receptor-related protein, and VLDL receptor. It also is the major plasma inhibitor of cholesteryl ester transfer protein and appears to interfere directly with fatty acid uptake. ApoC-I causes hypertriglyceridemia by inhibition of the lipoprotein lipase-dependent triglyceride-hydrolysis pathway. On the other hand, ApoC-I is capable of activating lecithin cholesterol acyl transferase that esterifies cholesterol and produces the formation of the mature HDL.