More info
Assay range | 7.81-250 ng/ml |
Sensitivity | 7.8 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, CSF or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoB standard: 1 vial
2. One 96-well plate coated with Human ApoB Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoB Ab (60x): 120 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein B (ApoB) is a 550 kDa palmitoylated and glycosylated member of the large lipid transfer (LLT) superfamily. It is a major protein component of chylomicron, verylow density lipoprotein (VLDL), and low density lipoprotein (LDL) particles. In humans, ApoB100 (the mature full-length of ApoB) is the predominant form expressed in the liver while ApoB48 corresponding
to the N-terminal 48% of ApoB100 predominates in the small intestine. Human ApoB (ApoB100) shares approximately 70% amino acid (aa) sequence identity with comparable regions of mouse and rat ApoB. The levels of secreted ApoB directly correlate with circulating serum cholesterol levels and is consistently associated with increased mortality in type 1 diabetes. Therefore, the determination of circulating ApoB levels is an important indicator of risk assessment for cardiovascular disease, insulin resistance, and metabolic syndrome.