More info
Assay range | 1.875-120 ng/ml |
Sensitivity | 1.8 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoA2 standard: 1 vial
2. One 96-well plate coated with Human ApoA2 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoA2 Ab (70x): 105 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein A-II (ApoA-II), also known as Apolipoprotein A2 (ApoA2), is the second most abundant apolipoproteins in human plasma HDL, comprising about 25% of the protein mass. Synthesized by the liver and intestine as a 100 amino acid (aa) precursor protein, ApoA-II is processed to 77 aa mature protein. In plasma, ApoA-II exists as a monomer, homodimer of 17.4 kDa, or heterodimer with ApoE and ApoD. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% -66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.
It has been reported that ApoA-II plays roles in HDL remodeling, cholesterol efflux, modulating HDL interaction with enzymes and receptors, triglyceride metabolism, and atherosclerosis. ApoA-II is inversely associated with risk of future coronary artery disease. In addition, serum levels of an isoform of ApoA-II have been identified as a potential marker for prostate cancer.