More info
Assay range | 3.125 to 200 ng/ml |
Sensitivity | 3.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Body fluids or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoA1 standard: 1 vial
2. One 96-well plate coated with Human ApoA1 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoA1 Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein A-I, also known as ApoA1, is a protein encoded by APOA1 gene in humans. ApoA1 is synthesized as a 267 amino acid (aa) precursor containing a signal peptide and a short propeptide. ApoA1 is the major component of serum HDL and is produced by the liver and small intestine. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.
ApoA1 plays an important role in promoting fat efflux, including cholesterol, from tissues to the liver for excretion. It also is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in APOA1 gene are associated with HDL deficiencies and with systemic non-neuropathic amyloidosis. The plasma level of ApoA-I is one of the best indicators of susceptibility to cardiovascular disease.